Lentivirus Vector Services
Custom and ready-to-use lentiviral vectors for efficient, stable gene delivery.
Trusted Vectors for Gene Therapy, Immunotherapy & Stem Cell Studies
Tailored Genes provides second and third-generation lentiviral vectors optimized for safe, efficient gene delivery into both dividing and non-dividing cells. Ideal for long-term expression, our vectors support applications in gene therapy, stem cell research, and immunotherapy. Produced under Public Health Agency of Canada guidelines, our vectors are manufactured using HEK293T cells and endotoxin-free plasmids for high purity and safety. We also offer comprehensive QC analytics including titration, vector copy number, and expression profiling — so you get data you can trust, from preclinical to clinical stages.
Why Choose Lentiviral Vectors?
Lentiviral vectors are a powerful and reliable tool for delivering genes into both dividing and non-dividing cells. Their ability to integrate into the host genome makes them ideal for achieving stable, long-term gene expression in even the most challenging cell types.
Long-Term Expression
Integrated delivery ensures consistent gene expression over time
Broad Cell Compatibility
Efficiently transduce primary cells, stem cells, and organoids
Flexible Vector Design
Supports inducible, dual-promoter, and CRISPR applications
High Transduction Efficiency
Achieve strong expression with lower viral input
Clinically Relevant System
Trusted in preclinical studies and gene therapy research
AAV Services
Custom Lentiviral Vectors Design
We design and produce high-quality, third-generation lentiviral vectors tailored to your gene expression goals. Services include:
01.
Custom promoter and marker selection (e.g., CMV, EF1α, eGFP, PuroR, etc.)
02.
Single or dual promoter configurations
03.
Inducible and CRISPR-compatible systems
04.
VSV-G pseudotyping for broad tropism
05.
Other retroviral envelope for targeting T-cells and NK-cells.
High-Titer, High-Purity Lentivirus Production
Our ultrafiltration process ensures titers of 10⁸–10⁹ TU/mL with near-clinical grade purity — ideal for:
- Hematopoietic stem cells
- Primary cells
- iPSCs
- Organoids
Ready-to-Use Lentivirus Vectors
Skip the production step with our pre-made LVVs, delivered in 100 µL aliquots at 10⁸ IU/mL. Available with EF1α promoter and ready-to-go reporters:
- eGFP
- BFP
- mPlum
- mNeptune
- Firefly luciferase
- mCherry
- eYFP
- dTomato
Custom promoter configurations available upon request. Available promoters include CMV, hPGK, hSyn.
FAQs
Frequently Asked Questions
Got questions? We’ve got answers. Browse through the most common questions to learn more about how we work, what we offer, and how we can help.
We predominantly manufacture 3rd Generation LV vectors. These 3rd generation vectors are manufactured by transfection of producer cells with 4 expression plasmids, a LV gag-pol expression plasmid, a LV Rev expression plasmid, a VSV-G envelope expression plasmid and an expression plasmid containing the gene of interest.
We can also manufacture 2nd Generation lentivirus vectors if requested. The 2nd generation vectors are produced by transfection of producer cells with 3 expression plasmids; a LV gag-pol-rev-tat expression plasmid, a VSV-G envelope expression plasmid, and an expression plasmid containing the gene of interest.
The MOI (Multiplicity of Infection) represents the ratio of the number of infectious units of virus vector per target cell.
For example, an MOI of 10 refers to 10 infectious units of vector per target cell. Therefore, if you need to infect 100,000 cells, you need to add 1,000,000 viral particles.
True infectious vector titers are determined by Absolute Q digital PCR.
For LV vector titration, genomic DNA isolated from target cells transduced with LV vector, is subjected to Absolute Q digital PCR to determine the number of integrated LV vector genomes using primer and probe specific to the vector sequence. LV vector titers are reported as IU/mL, where IU is an infectious unit and represents the number of infective viral particles.
LV vectors should be stored immediately at -80°C. All our vectors have a shelf-life of one year without loss in titer. In our tests we have observed a 10% loss in virus titer when vectors are stored for two years.
We recommend not to freeze-thaw your viral vector preparations multiple times. In our test studies we have found that there is an approximate 15% loss in vector titer from a single freeze-thaw. If you plan to use the virus for multiple experiments, thaw the viral preparation on ice, remove the desired volume for use in experiment, aliquot the remaining viral vector preparation into smaller aliquots, and snap-freeze aliquots in dry ice before storing at -80°C.
The maximum size of sequence that can be accommodated between LTRs is 9.0kb. Taking into consideration the LV packaging sequence, RRE and cPPT required in the transgene expression vector, only 7.5kb of promoter-transgene sequences can be added to the vector. There is an inverse relationship between the size of the transgene vector and LV vector titer (Systematic Determination of the Packaging Limit of Lentiviral Vectors Mukesh Kumar, Brian Keller, NdeyeMakalou, Richard E. Sutton Human Gene Therapy. 2001, 12(15): 1893-1905).
LV vectors
Dulbecco’s Modified Eagle Medium.
Lentivirus (LV) vectors
We provide functional titers ie number of infectious units (IU) per mL which are more relevant than genomic copy/mL or total virus particles/mL. We provide 10⁸ IU/mL and 10⁹ IU/mL based on customer’s needs. Virus titers are dependent on the size and nature of the transgene expression construct. The infectious titer of each viral preparation is determined by Absolute Q digital PCR or by flow cytometry if a fluorescent gene is expressed. Titers are reported in the Certificate of Analysis sent with each shipment.
Why Choose Us for Lentivirus Vectors?
At Tailored Genes, we’re committed to delivering high-quality Lentivirus Vectors with speed, reliability, and personalized support. All of our vectors are manufactured in North America, ensuring compliance with stringent quality standards. Whether you need small-scale production in just two weeks or large-scale batches in four to five weeks, our streamlined process delivers results fast. We also back every order with a satisfaction guarantee—if something isn’t right, we’ll work closely with you to make it right.
- North American Manufacturing – All vectors are produced in North America, ensuring strict quality standards.
- Fast Turnaround Times – 2 weeks for small-scale, 4-5 weeks for large-scale production.
- Satisfaction Guarantee – We follow up after delivery to ensure the vectors meet your expectations. If issues arise, we’ll work with you to resolve them.
Our Solutions
Explore More Manufacturing Solutions
Expertly crafted virus vectors and cell line solutions—powering research from bench to breakthrough.
Adeno-Associated Virus (AAV)
High titer (10¹³ and 10¹⁴ GC/mL) and ultra pure AAV vectors.
Stable Cell Lines
Overexpress or repress gene expression in the cell line of your choice.
Lentivirus Vectors
High titer (10⁸–10⁹ IU/mL) and high purity LV vectors.
