LV and AAV vectors should be stored immediately at -80°C. All our vectors have shelf-life of one year without loss in titer. In our tests we have observed a 10% loss in virus titer when vectors are stored for two years.
We recommend not to freeze-thaw your viral vector preparations multiple times. In our test studies we have found that there is an approximate 15% loss in vector titer from a single freeze-thaw. If you plan to use the virus for multiple experiments, thaw the viral preparation on ice, remove the desired volume for use in experiment, aliquot the remaining viral vector preparation into smaller aliquots, and snap-freeze aliquots in dry ice before storing at -80°C.